Boithuto ba rona bo bonts'a hore Ganoderma lucidum/reishi/lingzhi e bonts'a thepa ea antitumor ho lisele tsa osteosarcoma in vitro.Ho ile ha fumanoa hore Ganoderma lucidum e thibela ho hōla ha lisele tsa kankere ea matsoele le ho falla ka ho hatella pontšo ea Wnt / β-catenin.E hatella mofets'e oa matšoafo ka ho sitisoa ha likhomaretsi tse tsepamisitsoeng le ho kenngoa ha MDM2-mediated Slug degradation.Ganoderma lucidum e thibela mofetše oa matsoele ka ho theola tsela ea PI3K/AKT/mTOR, Ganoderma lucidum e bapala karolo ea antitumor liseleng tse matla tsa leukemia ka ho thibela tsela ea MAPK.
CCK-8 le liteko tsa tlhahiso ea kolone, bakeng sa ho lekola phello ea Ganoderma lucidum ho osteosarcoma cell line viability le ho ata, li bonts'itse hore Ganoderma lucidum e hatella ho ata ha lisele tsa MG63 le U2-OS ka mokhoa o itšetlehileng ka nako le mahloriso, mme e fokotsa bokhoni ba lisele ho etsa kolone.
Ganoderma lucidum e laola polelo ea liphatsa tsa lefutso tsa proapoptotic, 'me tlhahlobo ea cytometry ea phallo e bontšitse hore apoptosis ea MG63 le lisele tsa U2-OS e eketseha ka mor'a phekolo ea Ganoderma lucidum.Ho falla ha lisele ke motheo oa mefuta e fapaneng ea boitšoaro ba baeloji, ho kenyelletsa angiogenesis, pholiso ea maqeba, ho ruruha, le metastasis ea mofetše.Ganoderma lucidum e fokotsa ho falla le ho hlaseloa ha lisele tse peli tsa lisele le ho thibela ho ata, ho falla le ho futuhela, 'me e baka apoptosis ea lisele tsa osteosarcoma.
Letšoao la Aberrant Wnt / β-catenin le amana haufi-ufi le ho thehoa, metastasis, le apoptosis ea mefuta e mengata ea kankere, ka ho phahama ha pontšo ea Wnt / β-catenin ho hlokomeloa ho osteosarcoma.
Phuputsong ena, litlhahlobo tsa motlalehi oa li-dual-luciferase li bontšitse hore phekolo ea Ganoderma lucidum e thibela pontšo ea CHIR-99021-activated Wnt / β-catenin.Sena se boetse se pakoa ke pontšo ea rona ea hore ho ngoloa ha liphatsa tsa lefutso tsa Wnt, tse kang LRP5, β-catenin, cyclin D1, le MMP-9, li thibetsoe ha lisele tsa osteosarcoma li tšoaroa ka Ganoderma lucidum.
Liphuputso tse fetileng li bontšitse ka lisampole tsa kliniki hore LRP5 e laoloa ho osteosarcoma e amanang le lisele tse tloaelehileng, le polelo ea LRP5 e amanang le lefu la metastatic le ho phela ho se nang maloetse, ho etsa hore LRP5 e be sepheo sa phekolo ea osteosarcoma.
β-catenin ka boeona ke sepheo sa bohlokoa tseleng ea pontšo ea Wnt / β-catenin, 'me polelo ea β-catenin ho osteosarcoma e eketseha haholo.Ha β-catenin e fetela khubung ho tloha cytoplasm, e kenya tšebetsong polelo ea liphatsa tsa lefutso tsa eona tse tlase, tse kenyelletsang cyclin D1, C-Myc, le MMPs.
Myc ke e 'ngoe ea li-proto-oncogenes tse kholo' me e phetha karolo ea bohlokoa ho laola ts'ebetso, ho ngoloa le ho thibela tlhahiso ea liphatsa tsa lefutso. Ho tlalehiloe hore ho hatelloa ha C-Myc oncogene ho etsa hore botsofali bo tsofale le apoptosis ea mefuta e mengata ea lisele tsa tumor, ho akarelletsa le lefu la osteosarcoma.
Cyclin D1 ke selefouno ea bohlokoa ea potoloho ea lisele tsa G1 mme e potlakisa phetoho ea karolo ea G1 / S.Polelo e feteletseng ea cyclin D1 e ka khutsufatsa potoloho ea lisele mme ea khothaletsa ho ata ha lisele ka potlako mefuteng e fapaneng ea hlahala.
MMP-2 le MMP-9 ke li-stromelysins tse nang le bokhoni ba ho theola likarolo tsa matrix tsa extracellular, e leng tšobotsi ea bohlokoa bakeng sa angiogenesis ea tumor le tlhaselo.
Sena se fana ka maikutlo a hore liphatsa tsa lefutso tsa Wnt/β-catenin li phetha karolo ea bohlokoa tsoelo-peleng ea osteosarcoma, le hore ho thibela li-node tsena tsa matšoao ho ka ba le phello e tsotehang ea phekolo.
Ka mor'a moo, re ile ra lemoha polelo ea mRNA le protheine ea liphatsa tsa lefutso tse amanang le pontšo ea Wnt/β-catenin ka PCR le blotting ea bophirimela.Meleng ka bobeli ea lisele, Ganoderma lucidum e thibetse polelo ea liprotheine tsena le liphatsa tsa lefutso.Liphetho tsena li tsoela pele ho bontša hore Ganoderma lucidum e thibela pontšo ea Wnt/β-catenin ka ho lebisa tlhokomelo LRP5, β-catenin, C-Myc, cyclin D1, MMP-2, le MMP-9.
E-cadherin ke transmembrane glycoprotein e hlahisoang haholo liseleng tsa epithelial 'me e hokahanya ho khomarela pakeng tsa lisele tsa epithelial le lisele tsa stromal.Ho hlakoloa kapa ho lahleheloa ke polelo ea E-cadherin ho lebisa tahlehelong kapa ho fokotseng ho khomarela lipakeng tsa lisele tsa hlahala, ho nolofalletsa lisele tsa hlahala hore li tsamaee habonolo, ebe li etsa hore hlahala e kenelle, e hasane, ebe e metastasize.Phuputsong ena, re fumane hore Ganoderma lucidum e ka laola E-cadherin, kahoo e hanyetsa phenotype ea Wnt / β-catenin-mediated ea lisele tsa osteosarcoma.
Qetellong, liphetho tsa rona li bonts'a hore Ganoderma lucidum e thibela pontšo ea osteosarcoma Wnt / β-catenin mme qetellong e lebisa ho fokotseha ha tšebetso ea lisele tsa osteosarcoma.Liphuputso tsena li fana ka maikutlo a hore Ganoderma lucidum e kanna ea ba sesebelisoa sa kalafo se sebetsang le se sebetsang bakeng sa kalafo ea osteosarcoma, lihlahisoa tse amanang le tsona li kenyelletsa.ganoderma lucidum spore oil softgels/reishi spore oil li-softgels,ganoderma lucidum spore powder/reishi spore powder
Nako ea poso: Apr-18-2022